Cytogenetic Laboratory Management

Author: Susan Mahler Zneimer
Publisher: John Wiley & Sons
ISBN: 9781119069775
Release Date: 2017-01-27
Genre: Science

Cytogenetic Laboratory Management: Chromosomal, FISH and Microarray-Based Best Practices and Procedures is a practical guide that describes how to develop and implement best practice processes and procedures in the genetic laboratory setting. The text first describes good laboratory practices, including quality management, design control of tests and FDA guidelines for laboratory developed tests, and pre-clinical validation study designs. The second focus of the book describes best practices for staffing and training, including cost of testing, staffing requirements, process improvement using Six Sigma techniques, training and competency guidelines and complete training programs for cytogenetic and molecular genetic technologists. The third part of the text provides step-wise standard operating procedures for chromosomal, FISH and microarray-based tests, including pre-analytic, analytic and post-analytic steps in testing, and divided into categories by specimen type, and test-type. All three sections of the book include example worksheets, procedures, and other illustrative examples that can be downloaded from the Wiley website to be used directly without having to develop prototypes in your laboratory. Providing both a wealth of information on laboratory management and molecular and cytogenetic testing, Cytogenetic Laboratory Management will be an essential tool for laboratorians world-wide in the field of laboratory testing and genetics testing in particular. This book gives the essentials of: Developing and implementing good quality management programs in laboratories Understanding design control of tests and pre-clinical validations studies and reports FDA guidelines for laboratory developed tests Use of reagents, instruments and equipment Cost of testing assessment and process improvement using Six Sigma methodology Staffing training and competency objectives Complete training programs for molecular and cytogenetic technologists Standard operating procedures for all components of chromosomal analysis, FISH and microarray testing of different specimen types This volume is a companion to Cytogenetic Abnormalities: Chromosomal, FISH and Microarray-Based Clinical Reporting. The combined volumes give an expansive approach to performing, reporting and interpreting cytogenetic laboratory testing and the necessary management practices, staff and testing requirements.

Cytogenetic Abnormalities

Author: S. Zneimer
Publisher: John Wiley & Sons
ISBN: 9781118412565
Release Date: 2014-08-21
Genre: Science

This guide discusses chromosomal abnormalities and how best to report and communicate lab findings in research and clinical settings. Providing a standard approach to writing cytogenetic laboratory reports, the guide further covers useful guidance on implementing International System for Human Cytogenetic Nomenclature in reports. Part one of the guide explores chromosomal, FISH, and microarray analysis in constitutional cytogenetic analyses, while part two looks at acquired abnormalities in cancers. Both sections provide illustrative examples of chromosomal abnormalities and how to communicate these findings in standardized laboratory reports.

The AGT Cytogenetics Laboratory Manual

Author: Marilyn S. Arsham
Publisher: John Wiley & Sons
ISBN: 9781119061229
Release Date: 2017-04-24
Genre: Science

Cytogenetics is a segment of the evolving cytogenomic branch of genetics that explores the genetic makeup of a cell, in particular chromosomes both in metaphase and in interphase cycles, as well as at the molecular level. It utilizes an assortment of procedures to illuminate both the full complement of chromosomes and a targeted region within a specific chromosome. These investigative tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structures, and molecular probes, such as fluorescence in situ hybridization and chromosome microarray analysis, which employ a variety of methods to highlight a region as small as a single, specific genetic sequence under investigation. The AGT Cytogenetics Laboratory Manual, Fourth Edition offers a comprehensive treatment of the diagnostic tests offered by this clinical laboratory environment and explains the scientific theories behind those procedures. One of the most valuable assets is its rich compilation of laboratory-tested protocols currently being used in leading laboratories, along with practical advice for nearly every area of interest to cytogeneticists. In addition to covering essential topics that have been the backbone of cytogenetics for over 60 years, such as the basic components of a cell, use of a microscope, human tissue processing for cytogenetic analysis (prenatal, constitutional, and neoplastic), laboratory safety, and the mechanisms behind chromosome rearrangement and aneuploidy, this edition introduces new and expanded chapters that reflect the intuitive experience of its authors. Some of these new topics include a unique collection of chromosome heteromorphisms; clinical examples of genomic imprinting; an example-driven overview of chromosomal microarray; mathematics specifically geared for the cytogeneticist; usage of ISCN's cytogenetic language; tips for laboratory management; examples of laboratory information systems; a collection of Internet and library resources; and a special chapter on animal chromosomes for the research and zoo cytogeneticist. The range of topics is thus broad yet comprehensive, offering the student with a resource that teaches the procedures performed in the cytogenetics laboratory environment, and the laboratory professional with a peer-reviewed reference that explores the multiple facets behind each of these procedures. Topic coverage is rich in detail, yet presented with the student in mind, making it a useful resource for researchers, clinicians, and laboratory professionals, as well as students in a university or medical school that offers introductory or laboratory-related courses exploring cytogenetics' role within medical genetics. Reviews for the third edition: "A collection of protocols and explanations for cytogenetic techniques from the common to the peculiar." "An excellent book. Looking forward to an updated version."

The Principles of Clinical Cytogenetics

Author: Steven L. Gersen
Publisher: Springer Science & Business Media
ISBN: 9781441916884
Release Date: 2013-03-26
Genre: Medical

In this thoroughly revised and expanded third edition of the highly praised classic, The Principles of Clinical Cytogenetics, a panel of hands-on experts update their descriptions of the basic concepts and interpretations involved in chromosome analysis to include the many advances that have occurred in the field. Among the highlights are a full chapter devoted to advances in chromosome microarray, soon to become a standard of care in this field, as well as an update on chromosome nomenclature as reflected in ISCN 2009. Other features include an update on automation to reflect the current state of the art, an update on hematopoietic neoplasms to reflect the new WHO guidelines, and updates on all regulatory changes that have been implemented. Cutting edge and readily accessible, The Principles of Clinical Cytogenetics, Third Edition offers physicians who depend on the cytogenetics laboratory for the diagnosis of their patients, students in cytogenetics programs, graduate and medical students studying for board examinations, cytogenetics technologists, and cytogeneticists a clear understanding of what happens in the cytogenetics laboratory to facilitate accurate and timely diagnoses.

Molecular Diagnostics

Author: George P. Patrinos
Publisher: Academic Press
ISBN: 9780128029886
Release Date: 2016-10-27
Genre: Medical

Molecular Diagnostics, Third Edition, focuses on the technologies and applications that professionals need to work in, develop, and manage a clinical diagnostic laboratory. Each chapter contains an expert introduction to each subject that is next to technical details and many applications for molecular genetic testing that can be found in comprehensive reference lists at the end of each chapter. Contents are divided into three parts, technologies, application of those technologies, and related issues. The first part is dedicated to the battery of the most widely used molecular pathology techniques. New chapters have been added, including the various new technologies involved in next-generation sequencing (mutation detection, gene expression, etc.), mass spectrometry, and protein-specific methodologies. All revised chapters have been completely updated, to include not only technology innovations, but also novel diagnostic applications. As with previous editions, each of the chapters in this section includes a brief description of the technique followed by examples from the area of expertise from the selected contributor. The second part of the book attempts to integrate previously analyzed technologies into the different aspects of molecular diagnostics, such as identification of genetically modified organisms, stem cells, pharmacogenomics, modern forensic science, molecular microbiology, and genetic diagnosis. Part three focuses on various everyday issues in a diagnostic laboratory, from genetic counseling and related ethical and psychological issues, to safety and quality management. Presents a comprehensive account of all new technologies and applications used in clinical diagnostic laboratories Explores a wide range of molecular-based tests that are available to assess DNA variation and changes in gene expression Offers clear translational presentations by the top molecular pathologists, clinical chemists, and molecular geneticists in the field

Human Stem Cell Manual

Author: Suzanne Peterson
Publisher: Academic Press
ISBN: 9780123854742
Release Date: 2012-10-22
Genre: Science

This manual is a comprehensive compilation of "methods that work" for deriving, characterizing, and differentiating hPSCs, written by the researchers who developed and tested the methods and use them every day in their laboratories. The manual is much more than a collection of recipes; it is intended to spark the interest of scientists in areas of stem cell biology that they may not have considered to be important to their work. The second edition of the Human Stem Cell Manual is an extraordinary laboratory guide for both experienced stem cell researchers and those just beginning to use stem cells in their work. Offers a comprehensive guide for medical and biology researchers who want to use stem cells for basic research, disease modeling, drug development, and cell therapy applications. Provides a cohesive global view of the current state of stem cell research, with chapters written by pioneering stem cell researchers in Asia, Europe, and North America. Includes new chapters devoted to recently developed methods, such as iPSC technology, written by the scientists who made these breakthroughs.

Advanced Building Technologies for Sustainability

Author: Asif Syed
Publisher: John Wiley & Sons
ISBN: 9780470546031
Release Date: 2012-07-03
Genre: Architecture

Practical solutions for sustainability In this timely guide, one of the world's leaders in advanced building technology implementation shows architects and engineers proven and practical methods for implementing these technologies in sustainably-designed buildings. Because of the very limited time architects are given from being awarded a project to concept design, this book offers clear and workable solutions for implementing solar energy, radiant heating and cooling floors, displacement ventilation, net zero, and more. It provides helpful tips and suggestions for architects and engineers to work together on implementing these technologies, along with many innovative possibilities for developing a truly integrated design. This book also explores and explains the many benefits of advanced technologies, including reduced greenhouse gas emissions, lower operating costs, noise reduction, improved indoor air quality, and more. In addition, Advanced Building Technologies for Sustainability: Offers detailed coverage of solar energy systems, thermal energy storage, geothermal systems, high-performance envelopes, chilled beams, under-floor air distribution, displacement induction units, and much more Provides case studies of projects using advanced technologies and demonstrates their implementation in a variety of contexts and building types Covers the implementation of advanced technologies in office towers, large residential buildings, hospitals, schools, dormitories, theaters, colleges, and more Complete with a clear and insightful explanation of the requirements for and benefits of acquiring the U.S. Green Building Council's LEED certification, Advanced Building Technologies for Sustainability is an important resource for architects, engineers, developers, and contractors involved in sustainable projects using advanced technologies.

Chasing the Heiress

Author: Rachael Miles
Publisher: Zebra Shout
ISBN: 9781420140897
Release Date: 2016-05-31
Genre: Fiction

“Intrigue, romance, adventure—Chasing the Heiress has it all. I’m a Rachel Miles fan!”—Cathy Maxwell, New York Times bestselling author "Her characters come alive with warmth and purpose." --Jodi Thomas, New York Times bestselling author Heiress On The Run Lady Arabella Lucia Fairborne has no need of a husband. She has a fine inheritance for the taking, a perfectly capable mind, and a resolve as tough as nails. But what she doesn't have is the freedom to defy her cousin's will--and his will is to see her married immediately to the husband of his choosing. So is it any wonder that she dresses herself as a scullery maid and bolts into the night? Colin Somerville's current mission for the home office is going poorly. Who would have expected otherwise for a rakish spy tasked with transporting a baby to the care of the royal palace. But when, injured and out of ideas, Colin stumbles upon a beautiful maid who knows her way around a sickroom, it seems salvation has arrived. Until he realizes that though Lucy may be able to help him survive his expedition, he may not escape this ordeal with his heart intact…

Molecular Biomethods Handbook

Author: John M. Walker
Publisher: Springer Science & Business Media
ISBN: 1603273751
Release Date: 2008-11-04
Genre: Science

Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.

Measurement and Evaluation in Human Performance

Author: James R. Morrow
Publisher: Human Kinetics 1
ISBN: 0736065032
Release Date: 2006-06-01
Genre: Education

Measurement and Evaluation in Human Performance, Third Edition,offers unmatched, in-depth instruction in measurement and evaluation techniques. Thoroughly revised and updated, the third edition features a new section on epidemiology and further develops international perspectives. This edition also features improved readability in measurement statistics and enhanced efficiency in solving measurement and evaluation problems through the use of the Statistical Package for the Social Sciences (SPSS). Additional features that are new to this edition: -Revised first chapter that lays the groundwork for improved learning throughout the text -Inclusion of a specific downloadable data set that is used as an example in many chapters -Removal of outdated and complicated statistical techniques, eliminating the need for a background in higher-level mathematics -Additional computer tasks in each chapter that tie earlier learning to specific applications -Decision scenarios similar to those made by human performance professionals to help students apply decision making skills to practical, real-life problems The text also increases the number of problem sets and places a greater emphasis on student-friendly learning through its learning aids, including chapter objectives, measurement and evaluation challenges, highlight features, mastery items, and new computer tasks for each chapter. Measurement and Evaluation in Human Performance, Third Edition,will appeal to students by engaging them in the material to increase their comprehension, and to professionals through its depth of information and ease in locating it. Includes an online study guide! To assist students using the text, Measurement and Evaluation in Human Performance, Third Edition, has a companion online student study guide that allows students to interactively practice, review, and develop their understanding of measurement and evaluation processes so that they can make strong grades and valid decisions. Students will receive free access to the study guide with the purchase of a new text, or it may be purchased as a separate component The online study guide is designed to help students learn, understand, and practice the main concepts of each chapter. Students will find selected answers to mastery items from the text, homework problems, selected homework answers, data matrixes to download, and multiple choice quizzes designed to test their knowledge of the textbook material. They will also find lecture outlines created by the authors to help them learn the key concepts, as well as links to related sites on the Web and a variety of test tips.

Laboratory Guide to the Methods in Biochemical Genetics

Author: Beat Thöny
Publisher: Springer Science & Business Media
ISBN: 9783540766988
Release Date: 2008-05-31
Genre: Medical

This manual deals specifically with laboratory approaches to diagnosing inborn errors of metabolism. The key feature is that each chapter is sufficiently detailed so that any individual can adopt the described method into their own respective laboratory.

Manual of Cytogenetics in Reproductive Biology

Author: Pankaj Talwar
Publisher: JP Medical Ltd
ISBN: 9789350909928
Release Date: 2014-02-28
Genre: Medical

Examines the diagnostic role of cytogenetics in improving the outcome of assisted reproductive technologies (ART). Covers basics of genetics, followed by investigative cytogenetics, applied cytogenetics, recent advances, preimplantation and prenatal cytogenetics.

The Genetic Basis of Haematological Cancers

Author: Alistair G. Reid
Publisher: John Wiley & Sons
ISBN: 9780470979389
Release Date: 2016-05-02
Genre: Medical

Written by a team of international experts, this book provides an authoritative overview and practical guide to the molecular biology and genetic basis of haematologic cancers including leukemia. Focusing on the importance of cytogenetics and related assays, both as diagnostic tools and as a basis for translational research, this is an invaluable guide for basic and clinical researchers with an interest in medical genetics and haemato-oncology. The Genetic Basis of Haematological Cancers reviews the etiology and significance of genetic and epigenetic defects that occur in malignancies of the haematopoietic system. Some of these chromosomal and molecular aberrations are well established and already embedded in clinical management, while many others have only recently come to light as a result of advances in genomic technology and functional investigation. The book includes seven chapters written by clinical and academic leaders in the field, organised according to haematological malignancy sub-type. Each chapter includes a background on disease pathology and the genetic abnormalities most commonly associated with the condition. Authors present in-depth discussions outlining the biological significance of these lesions in pathogenesis and progression, and their use in diagnosis and monitoring response to therapy. The current or potential role of specific abnormalities as novel therapeutic targets is also discussed. There is also a full colour section containing original FISH, microarrays and immunostaining images.

Medical and Health Genomics

Author: Dhavendra Kumar
Publisher: Academic Press
ISBN: 9780127999227
Release Date: 2016-06-04
Genre: Science

Medical and Health Genomics provides concise and evidence-based technical and practical information on the applied and translational aspects of genome sciences and the technologies related to non-clinical medicine and public health. Coverage is based on evolving paradigms of genomic medicine—in particular, the relation to public and population health genomics now being rapidly incorporated in health management and administration, with further implications for clinical population and disease management. Provides extensive coverage of the emergent field of health genomics and its huge relevance to healthcare management Presents user-friendly language accompanied by explanatory diagrams, figures, and many references for further study Covers the applied, but non-clinical, sciences across disease discovery, genetic analysis, genetic screening, and prevention and management Details the impact of clinical genomics across a diverse array of public and community health issues, and within a variety of global healthcare systems

Cancer Cytogenetics

Author: John Swansbury
Publisher: Springer Science & Business Media
ISBN: 9781592593637
Release Date: 2003
Genre: Medical

A collection of key cytogenetic and FISH techniques used by modern clinical laboratories in the genetic analysis of human malignancies. The book's practical advice and methods are suitable for use at every level of expertise, including fully established laboratories. Included are tutorials on the fundamentals of human karyotypes and chromosome analysis, as well as detailed discussions on how laboratories may optimally upgrade their repertoire of capabilities to include such newer complementary techniques as CGH, FISH, and M-FISH.