Mass Spectrometry of Non Covalent Complexes

Author: Christoph A. Schalley
Publisher: John Wiley & Sons
ISBN: 9780470131152
Release Date: 2009-09-08
Genre: Science

Details the many benefits of applying mass spectrometry tosupramolecular chemistry Except as a method for the most basic measurements, massspectrometry (MS) has long been considered incompatible withsupramolecular chemistry. Yet, with today's methods, the disconnectbetween these two fields is not warranted. Mass Spectrometry andGas-Phase Chemistry of Non-Covalent Complexes provides aconvincing look at how modern MS techniques offer supramolecularchemists a powerful investigatory toolset. Bringing the two fields together in an interdisciplinary manner,this reference details the many different topics associated withthe study of non-covalent complexes in the gas phase. The textbegins with brief introductions to supramolecular chemistry andsuch relevant mass spectrometric methods as ionization techniques,analyzers, and tandem MS experiments. The coverage continueswith: How the analyte's transition into the gas phase changes covalentbonding How limitations and pitfalls in analytical methods may producedata misinterpretations Artificial supramolecular aggregates and their examination Biomolecules, their complexes, and their examination After the general remarks making up the first section of thebook, the following sections describe specific experimentalprocedures and are illustrated with numerous examples and shorttutorials. Detailed citations end each chapter. Massspectrometrists, supramolecular chemists, students in these fields,and interested readers from other disciplines involving the studyof non-covalent bonds will all value Mass Spectrometry andGas-Phase Chemistry of Non-Covalent Complexes as an innovativeand practical resource.

Mass Spectrometry

Author: Edmond de Hoffmann
Publisher: John Wiley & Sons
ISBN: 9780470033104
Release Date: 2007-10-29
Genre: Science

The latest edition of a highly successful textbook, Mass Spectrometry, Third Edition provides students with a complete overview of the principles, theories and key applications of modern mass spectrometry. All instrumental aspects of mass spectrometry are clearly and concisely described: sources, analysers and detectors. Tandem mass spectrometry is introduced early on and then developed in more detail in a later chapter. Emphasis is placed throughout the text on optimal utilisation conditions. Various fragmentation patterns are described together with analytical information that derives from the mass spectra. This new edition has been thoroughly revised and updated and has been redesigned to give the book a more contemporary look. As with previous editions it contains numerous examples, references and a series of exercises of increasing difficulty to encourage student understanding. Updates include: Increased coverage of MALDI and ESI, more detailed description of time of flight spectrometers, new material on isotope ratio mass spectrometry, and an expanded range of applications. Mass Spectrometry, Third Edition is an invaluable resource for all undergraduate and postgraduate students using this technique in departments of chemistry, biochemistry, medicine, pharmacology, agriculture, material science and food science. It is also of interest for researchers looking for an overview of the latest techniques and developments.

New Methods for the Study of Biomolecular Complexes

Author: W. Ens
Publisher: Springer Science & Business Media
ISBN: 9789401590464
Release Date: 2013-03-09
Genre: Science

A NATO Advanced Research Workshop entitled New Methods for the Study of Molecular Aggregates was held at Tbe Lodge at Kananaskis Village, Alberta, Canada from 16 -20 June 1996. In fact the meeting was entirely concerned with the problem of analyzing biomolecular complexes, so the title of these proceedings has been altered to give a more precise description of the content. Tbe workshop was hosted by the time-of-flight group of the Department of Physics at the University of Manitoba, and was attended by 64 participants from around the world. '!\venty-one invited talks were given and 27 papers were presented as posters. Of the 48 contributions, 22 papers (12 orals, 10 posters) are included in these proceedings. Tbe subject of the conference was the investigation of noncovalent biomolecular complexes, with particular focus on the application of mass spectrometry to their characterization. '!\vo new ionization techniques introduced in the late 1980s, electrospray ionization (ES I) and matrix-assisted laser desorptionlionization (MALDI), resulted in a breakthrough in mass spectrometry, enabling its use in molecular weight and primary structure determination of biopolymers larger than 100 kDa. Recently it has been discovered that ESI mass spectrometry mayaiso be used to characterize complexes containing noncovalent interactions, thus opening new perspectives for supramolecular chemistry. ESI mass spectrometry has the advantage that the sampie is introduced from a homogenous solution which can be maintained at near physiological conditions of pR, concentration, and temperature.

Biological Mass Spectrometry

Author: A. L. Burlingame
Publisher: Gulf Professional Publishing
ISBN: 0121828077
Release Date: 2005
Genre: Science

This volume describes and integrates the techniques and fundamentals of more than a decade of revolutionary advances in both chromatographic and mass spectrometric technologies that have enabled the direct investigation of biomacromolecules per se and have provided the analytical power base to usher in the new fields of proteomics and systems biology. It also covers new biophysical applications such as H/D exchange for study of conformations, protein-protein and protein-metal and ligand interactions. Finally it describes atto-to-zepto-mole quantitation of 14C and 3H by accelerator mass spectrometry. *Part 1 of 2 volumes about Mass Spectrometry *Authoritative and comprehensive treatment of protein mass spectrometry in human cell biology *Presents fundamentals, techniques, instrumentation and bioinformatics *Provides an overview of proteomics, protein-protein and protein-ligand binding, and biophysical studies

Mass Spectrometry of Nucleosides and Nucleic Acids

Author: Joseph H. Banoub
Publisher: CRC Press
ISBN: 1420044036
Release Date: 2009-12-21
Genre: Science

Assembling the work of an international panel of researchers, Mass Spectrometry of Nucleosides and Nucleic Acids summarizes and reviews the latest developments in the field and provides a window on the next generation of analysis. Beginning with an overview of recent developments, the book highlights the most popular ionization methods and illustrates the diversity of strategies employed in the characterization and sequencing of DNA and RNA oligomers, nucleosides, nucleotides, and adducts. It describes studies performed on deoxyinosine and its analogues and provides an introduction to tandem mass spectrometry (MS/MS). Next, the contributors examine mass spectrometric application in the study of cyclic nucleotides in biochemical signal transduction. They analyze urinary modified nucleosides and explore DNA adducts. They discuss isotope labeling of DNA-mass spectrometry (ILD-MS) and examine various uses of electrospray ionization mass spectrometry (ESI-MS). The book reviews recent progress in the direct MS characterization of noncovalent nucleic acid-protein complexes, explores the interaction and ionization of guanidine-derived compounds with highly acidic biomolecules, and examines quantitative identification of nucleic acids via signature digestion products detected using mass spectrometry. The book describes a direct-infusion ESI-MS approach that can serve as a screening technique for the presence of modified nucleosides from small RNAs. Lastly, it discusses the LC-MS/MS method for the in vitro replication studies on damage-containing DNA substrates, and concludes with an examination of the influence of metal ions on the structure and reactivity of nucleic acids. The exciting developments in mass spectrometry technology have fueled incredible advances in our understanding of nucleic acids and their complexes. The contributions presented in this volume capture the range of these advances, helping to inspire new findings and avenues of research.

Mass Spectrometry in Structural Biology and Biophysics

Author: Igor A. Kaltashov
Publisher: John Wiley & Sons
ISBN: 9780470937792
Release Date: 2012-04-03
Genre: Medical

With its detailed and systematic coverage of the current state of biophysical mass spectrometry (MS), here is one of the first systematic presentations of the full experimental array of MS-based techniques used in biophysics, covering both fundamental and practical issues. The book presents a discussion of general biophysical concepts and a brief overview of traditional biophysical techniques before outlining the more advanced concepts of mass spectrometry. The new edition gives an up-to-date and expanded coverage of experimental methodologies and a clear look at MS-based methods for studying higher order structures and biopolymers. A must for researchers in the field of biophysics, structural biology, and protein chemistry.

Mass Spectrometry of Proteins and Peptides

Author: John R. Chapman
Publisher: Springer Science & Business Media
ISBN: 9781592590452
Release Date: 2000
Genre: Science

New types of mass spectrometric instrumentation and new ionization techniques have dramatically extended the applicability of mass spectrometry to the analysis of proteins and peptides. In Mass Spectrometry of Proteins and Peptides, John R. Chapman recreates the success of his earlier acclaimed book (Protein and Peptide Analysis by Mass Spectrometry, 1996) with a major new collection of cutting-edge methods in this area. Contributed by well-established investigators, each chapter provides background information before guiding the researcher step-by-step through the experimental process, with detailed instructions to help assure experimental success. The applications covered range widely and include protein sequencing, higher-order structure determination, epitope mapping, kinetics, quantitation, glycosylation analysis, and bacterial typing. Authoritative and eminently practical, Mass Spectrometry of Proteins and Peptides demonstrates the full scope of this versatile analytical technique. All protein chemists and biochemists, as well as bioanalytical scientists, who want to exploit these powerful methods in their work, will find this book indispensable.

Mass Spectrometry in Biology Medicine

Author: A.L. Burlingame
Publisher: Springer Science & Business Media
ISBN: 9781592597192
Release Date: 1999-09-14
Genre: Science

Leading practitioners detail revolutionary new spectrometric techniques for the identification and covalent structural characterization of macromolecules, proteins, glycoconjugates, and nucleic acids. Based on the Fourth International Symposium on Mass Spectrometry in the Health and Life Sciences held in San Francisco in 1998, this invaluable book contains tested strategies for solving many significant biomedical research problems. The techniques use mass spectrometry, automated computer processing of spectral information, and gene, protein, and EST databases for genomic and proteomic correlations. Mass Spectrometry in Biology and Medicine offers a unique opportunity to explore and apply these new techniques of mass spectrometry that are revolutionizing the identification and structural characterization of proteins, carbohydrates, and nucleic acids.

Mass Spectrometry in Medicinal Chemistry

Author: Klaus Wanner
Publisher: John Wiley & Sons
ISBN: 9783527314560
Release Date: 2007-04-09
Genre: Medical

This first overview of mass spectrometry-based pharmaceutical analysis is the key to improved high-throughput drug screening, rational drug design and analysis of multiple ligand-target interactions. The ready reference opens with a general introduction to the use of mass spectrometry in pharmaceutical screening, followed by a detailed description of recently developed analytical systems for use in the pharmaceutical laboratory. Applications range from simple binding assays to complex screens of biological activity and systems containing multiple targets or ligands -- all highly relevant techniques in the early stages in drug discovery, from target characterization to hit and lead finding.